Application

Research Sub CategoryImmunological Signaling

Research CategoryApoptosis & Cancer

This Anti-MS4A4A Antibody, clone 3F2 is validated for use in Flow Cytometry for the detection of MS4A4A.

Flow Cytometry Analysis: A representative lot, when pre-conjugated with Alexa Fluor^® 647, detected subpopulations with surface MS4A4A immunoreactivity among the CD14+ and CD16+ cells within the gated monocytes population from isolated human peripheral blood leukocytes (Courtesy of Julie Deans, PhD, University of Calgary, Alberta, Canada).

Flow Cytometry Analysis: A representative lot, when pre-conjugated with Alexa Fluor™ 647, selectively detected MS4A4A immunoreactivity on alternatively activated (M2), but not classically activated (M1), macrophages generated in vitro from human peripheral blood monocytes (Courtesy of Julie Deans, PhD, University of Calgary, Alberta, Canada)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Membrane-spanning 4-domains subfamily A member 4A (UniProt Q96JQ5; also known as CD20 antigen-like 1, Four-span transmembrane protein 1) is encoded by the MS4A4A (also known as 4SPAN1, CD20L1, MS4A4) gene (ORF HDCME31P; Gene ID 51338) in human. Human MS4A4A belongs to the family of genes coding for the tetraspanning MS4A proteins that likely mediate cellular signaling by acting as cell-surface signaling molecules and intracellular adapter proteins, although their exact functions remain to be characterized. MS4A4A immunoreactivity can be detected among the CD14+ and CD16+ monocytes population from isolated human peripheral blood leukocytes, as well as on the surface of alternatively activated (M2), but not classically activated (M1), macrophages from human PBMCs. Tissue distribution by quantitative RT-PCR analysis revealed only detectable lung MS4A4A mRNA level among all non-hematopoietic tissues examined, while no MS4A4A transcript could be detected in B-cells isolated from normal blood or tonsil tissue. MS4A4A expression profiling involving 14 blood samples from chronic lymphocytic leukemia (CLL) patients identified one high level CLL sample. Human MS4A4A protein consists of four transmembrane domains (65-85, 99-119, 138-158, 180-200), having both its N- and C-terminal ends exposed intracellularly (a.a. 1-64, 201-239).80-200), having both its N- and C-terminal ends exposed intracellularly (a.a. 1-64, 201-239).

Immunogen

Epitope: Extracellular domain.

Human MS4A4A GFP fusion-expressing 300-19 mouse pre-B cells.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Flow Cytometry Analysis of MS4A4A-GFP expression in transfected BJAB Burkitt′s lymphoma cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected the expression of exogenously transfected human MS4A4A GFP fusion in BJAB Burkitt′s lymphoma cells.

Specificity

The exact location of the extracellular epitope or the reactivity of clone 3F2 toward the spliced isoform 2 (UniProt Q96JQ5-2) that lacks the second extracellular loop has not been determined.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

25.44 kDa (isoform 1) and 19.68 kDa (isoform 2) calculated.